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Research Update: The Effect Of Equine Bodyweight On Performance

A recent study conducted by scientists in Sweden and Iceland examined the effect of altered body weight and body fat content on exercise performance and recovery. Nine horses were divided into two groups, and changes in bodyweight and fat content were induced by feeding a high or restricted energy diet. After 36 days, horses were switched to the other diet and the study was repeated. In the last week of each diet, bodyweight and body condition score were recorded, body fat percentage was estimated using ultrasound, and a standardized incremental treadmill exercise test and competition-like field test were performed.

Bodyweight, body fat percentage, and body condition score were higher (5 to 8 percent) in high energy horses compared to horses fed the restricted energy diet. In the standardized exercise test, horses on the high energy diet performed more poorly and recovered more slowly compared to horses fed the restricted energy diet. Additionally, locomotion asymmetry was greater in horses fed the high energy diet compared to the restricted energy diet. These results confirmed that increased bodyweight and body fat in horses lowered physiological fitness, reduced symmetry, and decreased performance ability. For more information on this research, read the paper published in Physiological Reports.

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Should Exercising Horses Receive Supplemental Antioxidants?

Among many other benefits, regular exercise enhances antioxidant defenses in horses. During exercise, aerobic cells generate reactive oxygen species (ROS), which are unstable atoms or molecules produced during normal cell metabolism. An overabundance of ROS can crush antioxidant defenses, leading to detrimental effects on muscle function. In an effort to quell the damage caused by ROS, researchers set out to determine the effect of N-acetyl cysteine and coenzyme Q10 supplementation on ROS in skeletal muscle of exercising horses.

Seven Thoroughbreds were used in the study, all engaged in the same training program: galloped 1.5 miles on the track three days a week, and walked and trotted on an automated exerciser three days a week. Horses were allowed access to pasture four days each week, three hours on exercise days and eight hours on their rest day. Horses were fed timothy hay ad libitum and a performance concentrate based on weight three times a day. Horses were supplemented daily with 10 g of N-acetyl cysteine and 1.6 g of coenzyme Q10.

Thirty days after supplementation began, horses performed an exercise test that consisted of a half-mile breeze on a racetrack at top speed. A second exercise test was performed at 60 days, with the same experienced rider instructed to replicate the speed from the earlier exercise test.

Muscle samples were taken the day before both exercise tests and one hour after each exercise test. Concentrations of antioxidants, cysteine, and ROS were measured. Blood samples were pulled prior to the exercise test and 10 minutes, one hour, and four hours after the exercise test.

The researchers concluded that supplementation of exercising Thoroughbred horses with N-acetyl cysteine and coenzyme Q10 for 30 days influenced antioxidant status without detrimental effects on performance.

Athletic performance and muscle diseases such as myofibrillar myopathy, equine motor neuron disease, and vitamin E responsive myopathy are affected by antioxidant status. Kentucky Equine Research has formulated multiple antioxidants designed for equine athletes, notably MFM Pellet, a palatable source of N-acetyl cysteine; Nano-E, a natural-source, water-soluble vitamin E supplement, and Nano-Q10, a highly bioavailable form of coenzyme Q10.

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“We have already seen the impact of this research, and horses diagnosed with myofibrillar myopathy are now being supplemented with MFM Pellet,” explained Marisa Henry, the lead author on the research paper. “We have heard glowing reviews from the owners of horses who have seen much improvement with MFM Pellet!”

This new study represents another chapter in the longstanding association between Kentucky Equine Research, Stephanie Valberg, D.V.M., Ph.D., and her colleagues, including Henry. Valberg is the director of the Equine Neuromuscular Diagnostic Laboratory and Mary Anne McPhail Dressage Chair in Equine Sports Medicine at Michigan State University, Department of Large Animal Clinical Sciences.

^*Henry, M.L., D. Velez-Irizarry, J.D. Pagan, L. Sordillo, J. Gandy, and S.J. Valberg. 2021. The impact of N-acetyl cysteine and coenzyme Q10 supplementation on skeletal muscle antioxidants and proteome in fit Thoroughbred horses. Antioxidants 10:1739.

Article reprinted courtesy of Kentucky Equine Research (KER). Visit equinews.com for the latest in equine nutrition and management, and subscribe to The Weekly Feed to receive these articles directly (equinews.com/newsletters).

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Bone Adaptation In Growing Foals

Mechanical loading strengthens bone during growth, so exercise during childhood and adolescence has been shown to have lifelong benefits on skeletal health in humans. Similarly, exercised foals show greater bone size and strength, and resistance to bending and torsional distortion, compared to pasture-exercised control foals.¹

Because the fetlock joint must withstand incredible forces, high-performance horses, including Thoroughbred and Standardbred racehorses, often sustain injuries at this site. The joint connects the long and short pastern bones, known also as the first and second phalanx.

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A recent study of sound Standardbred foals, all trotting-bred, characterized how the long pastern bone adapts to changing loads during growth through the use of regular computed tomography (CT) scans during the first year of life. In addition to measuring strength and composition changes in the bone, researchers calculated strain energy density, a biomechanical metric of potential bone remodeling.²

Growth-related changes in mineral density and bone area at various bone points (distal epiphysis, mid-diaphysis, and proximal epiphysis) were assessed. These three points were further divided into four functional quadrants (dorsal, medial, lateral, palmar). Mineral density and bone area uniformly increased in the diaphysis and a portion of the proximal epiphysis, the medial quadrant, but not in the fracture-prone lateral quadrant. Strain energy density was constant during growth, indicating bone adaptation to standing quietly, the primary activity of Standardbred foals, according to prior observation by researchers, which revealed that foals between the ages of two and five months spent 80 percent of their time at a standstill.

In sum, “the work provides a baseline longitudinal characterization of normal remodeling of the equine forelimb first phalanx during the first year of life and its effect of strain energy density,” the researchers explained. “This is an essential prerequisite to making evidence-based recommendations for training regimens that may encourage bone growth in areas prone to fracture during development. A properly prepared musculoskeletal system may lead to fewer fractures, thus reducing the unnecessary wastage of equine athletes.”

In addition to exercise, proper nutrition of the mare throughout gestation and of the foal during all growth phases fosters future soundness. “Nutritional management of young horses starts with an appropriate, good-quality forage. Fortified concentrates can then be fed to meet energy, mineral, and vitamin shortfalls,” said Catherine Whitehouse, M.S., a nutrition advisor at Kentucky Equine Research.

“Aside from hay and concentrates, supplements designed to increase bone health can be used to support young athletes. Triacton, a supplemented formulated by Kentucky Equine Research, contains a novel source of calcium and an array of bone-building nutrients designed to increase bone density, with the ultimate goal of promoting long-term soundness,” she explained. “Research in weanlings, for example, showed that Triacton had a positive effect on density of the cannon bone.”

¹Firth, E.C., C.W. Rogers, P.R. van Weeren, A. Barneveld, C.W. McIlwraith, C.E. Kawcak, A.E. Goodship, and R.K.W. Smith. 2011. Mild exercise early in life produces changes in bone size and strength but not density in proximal phalangeal, third metacarpal and third carpal bones of foals. Veterinary Journal 190:383-389.

²Moshage, S.G., A.M. McCoy, J.D. Polk, and M.E. Kersh. 2019. Temporal and spatial changes in bone accrual, density, and strain energy density in growing foals. Journal of the Mechanical Behavior of Biomedical Materials 103.

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‘Accident-Prone’ Horses May Merit Additional Examination

Though horse owners often joke that their horses seem to be looking for ways to injure themselves, is there a point where a horse may be more than simply “accident prone?” Dr. Amelia Munsterman, Associate Professor of Large Animal Surgery and Emergency Medicine at Michigan State University, tells EQUUS magazine there may be some additional reasons a horse may seem intent to harm himself, including soreness, lameness and neurologic issues.

The first step in determining whether there's an underlying problem is to have the horse thoroughly checked by a veterinarian, including a focused neurologic exam. This exam will put the horse through a variety of movements to see if he knows where his limbs and body are in space. A lameness exam is also helpful as a horse that is sore may alter his gait and the way he moves, causing him to run into things.

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One other thing the vet should check is the horse's vision. Limited sight in one or both eyes will cause difficulty for a horse trying to navigate his surroundings.

If all of these tests yield no concrete answers, the horse may simply be very curious; adding toys to his stall may keep him out of trouble, Munsterman says. Ensuring he is in a regular exercise program can assist with channeling his extra energy, and spacing out feedings can also help keep him busy and out of trouble.